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ARRA CTOT/CTOT-C Mechanistic Studies in Transplantation

Working Group Group Leader/Contact SOPs Publications
HLA Antibody Elaine Reed
HLA Antibody Luminex SOP GenProbe

HLA Antibody Luminex SOP OneLambda
Comprehensive Assessment and Standardization of Solid Phase Multiplex-Bead Arrays for the Detection of Antibodies to HLA. Reed EF, Rao P, Zhang Z, Gebel H, Bray RA, Guleria I, Lunz J, Mohanakumar T, Nickerson P, Tambur AR, Zeevi A, Heeger PS, Gjertson D. Department of Pathology and Laboratory Medicine, University of California Los Angeles, Los Angeles, CA. American Journal of Transplantation. 2013 Jul; 13(7):1859-70
Cellular Assays: Elispot Peter Heeger
SOP CTOT ARRA Elispot Appendix Standardization and Cross Validation of Alloreactive IFNy ELISPOT Assays Within the Clinical Trials in Organ Transplantation Consortium. Ashoor I, Najafian N, Korin Y, Reed EF, Mohanakumar T, Ikle D, Heeger PS, Lin M. Transplantation Research Center, Renal Division, Brigham and Women's Hospital, Children's Hospital, Boston, MA. American Journal of Transplantation. 2013 Jul; 13(7):1871-9.
Molecular Testing Rob Fairchild
SOP Urine Collection and Processing

SOP RNA Isolation from Urine Pellet
Multicenter evaluation of a standardized protocol for noninvasive gene expression profiling. Keslar KS, Lin M, Zmijewska AA, Sigdel TK, Tran TQ, Ma L, Bhasin M, Rao P, Ding R, Iklé DN, Mannon RB, Sarwal MM, Strom TB, Reed EF, Heeger PS, Suthanthiran M, Fairchild RL. Department of Immunology, Cleveland Clinic, Cleveland, OH. American Journal of Transplantation. 2013 Jul; 13(7):1891-7.
Genomics Dan Salomon
RNA Purification and Expression Analysis Molecular classifiers for acute kidney transplant rejection in peripheral blood by whole genome gene expression profiling.
Kurian SM, Williams AN, Gelbart T, Campbell D, Mondala TS, Head SR, Horvath S, Gaber L, Thompson R, Whisenant T, Lin W, Langfelder P, Robison EH, Schaffer RL, Fisher JS, Friedewald J, Flechner SM, Chan LK, Wiseman AC, Shdban H, Mendez R, Heliman R, Abecassis MM, Marsh CL, Salomon DR Am J Transplant. 2014 May;14(5):1164-72. doi: 10.1111/ajt.12671. Epub 2014 Apr 11.
Cellular Assays: Flow Cytometry Kenneth Newell
None None
Pathology John Friedewald
None None
Infectious Disease Jay Fishman
Haemophilus influenzae opsono-killing assay v1.2
Strep Pneumo Opsonophagocytic Assay v1.3
SOP slides
Rychert et al 10
Proteomics Minnie Sarwal
None Optimization for peptide sample preparation for urine peptidomics. Sigdel TK, Nicora CD, Hsieh SC, Dai H, Qian WJ, Camp DG 2nd, Sarwal MM. Clin Proteomics. 2014 Feb 25;11(1):7.

This research was performed as part of an American Recovery and Reinvestment Act (ARRA) funded project under Award Number U0163594 (to P Heeger), from the National Institute of Allergy and Infectious Diseases. The work was carried out by members of the Clinical Trials in Organ Transplantation (CTOT) and Clinical Trials in Organ Transplantation in Children (CTOT-C) consortia. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute Of Allergy And Infectious Diseases or the National Institutes of Health.